Gi3 - TSH receptor complex model

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gi/o

DATE

2023-09-15

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.


Tertiary structure confidence

Chain pLDDT score avg
TSH 79.4
Gαi3 87.0
Very high (>90)
Confident (90-70)
Low (70-50)
Very low (<50)
Disordered

PTM Score: 0.7
iPTM Score: 0.7
PAE mean: 12.8


GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM3 R 519 3x50 G.H5 L 353 G.H5.25
Hydrophobic C-term L 741 - G.H3 N 256 G.H3.15
Van-der-waals C-term L 741 - G.H3 N 256 G.H3.15
Polar TM3 A 522 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 522 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 A 522 3x53 G.H5 N 347 G.H5.19
Polar TM3 R 519 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 519 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 519 3x50 G.H5 C 351 G.H5.23
Ionic ICL2 R 528 34x52 G.s2s3 D 193 G.s2s3.02
Polar ICL2 R 528 34x52 G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 R 528 34x52 G.s2s3 D 193 G.s2s3.02
Polar TM6 K 618 6x29 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 K 618 6x29 G.h4s6 D 315 G.h4s6.09
Van-der-waals TM6 K 618 6x29 G.h4s6 D 315 G.h4s6.09
Hydrophobic TM5 V 608 5x65 G.H5 I 344 G.H5.16
Hydrophobic ICL2 A 526 34x50 G.H5 I 343 G.H5.15
Van-der-waals ICL2 A 526 34x50 G.H5 I 343 G.H5.15
Hydrophobic C-term V 738 - G.H2 I 212 G.H2.08
Van-der-waals C-term V 738 - G.H2 I 212 G.H2.08
Polar ICL3 Y 613 - G.H5 A 338 G.H5.10
Hydrophobic ICL3 Y 613 - G.H5 A 338 G.H5.10
Polar TM6 R 625 6x36 G.H5 G 352 G.H5.24
Hydrophobic TM3 I 523 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 I 523 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL3 Y 613 - G.H5 D 337 G.H5.09
Hydrophobic TM6 L 629 6x40 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 629 6x40 G.H5 L 353 G.H5.25
Van-der-waals TM6 K 621 6x32 G.H5 Y 354 G.H5.26
Hydrophobic ICL2 D 530 34x54 G.hns1 K 32 G.hns1.03
Van-der-waals ICL2 D 530 34x54 G.hns1 K 32 G.hns1.03
Hydrophobic C-term I 742 - G.H2 K 209 G.H2.05
Van-der-waals C-term I 742 - G.H2 K 209 G.H2.05
Hydrophobic TM5 V 608 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM6 I 622 6x33 G.H5 L 348 G.H5.20
Polar C-term P 713 - G.h2s4 E 216 G.h2s4.02
Hydrophobic C-term P 713 - G.h2s4 E 216 G.h2s4.02
Van-der-waals C-term P 713 - G.h2s4 E 216 G.h2s4.02
Hydrophobic C-term L 741 - G.H3 I 253 G.H3.12
Polar TM6 R 625 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 R 625 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 R 625 6x36 G.H5 L 353 G.H5.25
Hydrophobic ICL2 L 529 34x53 G.hns1 K 32 G.hns1.03
Hydrophobic ICL2 M 527 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 M 527 34x51 G.S3 L 194 G.S3.01
Hydrophobic TM4 R 536 4x40 G.HN R 24 G.HN.48
Hydrophobic ICL2 A 526 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 A 526 34x50 G.H5 I 344 G.H5.16
Polar TM6 K 618 6x29 G.H5 Y 354 G.H5.26
Hydrophobic TM6 K 618 6x29 G.H5 Y 354 G.H5.26
Van-der-waals TM6 K 618 6x29 G.H5 Y 354 G.H5.26
Hydrophobic H8 T 682 8x47 G.H5 G 352 G.H5.24
Hydrophobic C-term V 711 - G.H3 W 258 G.H3.17
Van-der-waals C-term V 711 - G.H3 W 258 G.H3.17
Hydrophobic C-term L 741 - G.H3 S 252 G.H3.11
Van-der-waals C-term L 741 - G.H3 S 252 G.H3.11
Hydrophobic TM3 I 523 3x54 G.H5 N 347 G.H5.19
Aromatic ICL3 Y 613 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 Y 613 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 Y 613 - G.S6 Y 320 G.S6.02
Hydrophobic ICL2 M 527 34x51 G.H5 T 340 G.H5.12
Van-der-waals ICL2 M 527 34x51 G.H5 T 340 G.H5.12
Ionic C-term D 737 - G.H3 K 257 G.H3.16
Polar C-term D 737 - G.H3 K 257 G.H3.16
Polar C-term Q 709 - G.h3s5 T 260 G.h3s5.01
Van-der-waals C-term Q 709 - G.h3s5 T 260 G.h3s5.01
Hydrophobic C-term S 745 - G.H2 R 205 G.H2.01
Hydrophobic ICL2 L 529 34x53 G.S1 V 34 G.S1.02
Hydrophobic TM5 Y 601 5x58 G.H5 L 353 G.H5.25
Hydrophobic ICL2 L 529 34x53 G.hns1 A 31 G.hns1.02
Van-der-waals ICL2 L 529 34x53 G.hns1 A 31 G.hns1.02
Hydrophobic TM3 I 523 3x54 G.H5 I 344 G.H5.16
Ionic TM6 K 618 6x29 G.h4s6 E 318 G.h4s6.12
Polar TM6 K 618 6x29 G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL2 L 529 34x53 G.H5 I 343 G.H5.15
Hydrophobic TM6 I 622 6x33 G.H5 Y 354 G.H5.26
Ionic ICL2 K 532 34x56 G.H5 E 350 G.H5.22
Polar ICL2 K 532 34x56 G.H5 E 350 G.H5.22
Van-der-waals ICL2 K 532 34x56 G.H5 E 350 G.H5.22
Hydrophobic ICL2 L 529 34x53 G.S3 L 194 G.S3.01
Van-der-waals ICL2 L 529 34x53 G.S3 L 194 G.S3.01
Polar TM6 R 625 6x36 G.H5 Y 354 G.H5.26
Hydrophobic TM6 R 625 6x36 G.H5 Y 354 G.H5.26
Van-der-waals TM6 R 625 6x36 G.H5 Y 354 G.H5.26
Ionic TM4 R 534 4x38 G.HN E 28 G.HN.52
Polar TM4 R 534 4x38 G.HN E 28 G.HN.52
Hydrophobic TM4 R 534 4x38 G.HN E 28 G.HN.52
Van-der-waals TM4 R 534 4x38 G.HN E 28 G.HN.52
Hydrophobic TM6 M 626 6x37 G.H5 L 353 G.H5.25
Polar TM6 K 618 6x29 G.h4s6 K 314 G.h4s6.08
Polar ICL3 Y 613 - G.H5 F 334 G.H5.06
Hydrophobic ICL3 Y 613 - G.H5 F 334 G.H5.06
Van-der-waals ICL3 Y 613 - G.H5 F 334 G.H5.06
Hydrophobic C-term L 741 - G.h2s4 F 215 G.h2s4.01
Hydrophobic ICL2 M 527 34x51 G.H5 F 336 G.H5.08
Van-der-waals ICL2 M 527 34x51 G.H5 F 336 G.H5.08