ArrestinDb

oprx_human

NAME

4EA3

ORGANISM

Human (Homo sapiens)

PROTEIN NAME

oprx_human

SOURCE

SWISSPROT

SEQUENCE

Perspective: Wild-type Construct


N-term
M	E	P	L	F	P	A	P	F	W
10



E	V	I	Y	G	S	H	L	Q	G
20



N	L	S	L	L	S	P	N	H	S
30



L	L	P	P	H	L	L	L	N	A
40


							TM1
S	H	G	A	F	L	P	L	G	L
50



K	V	T	I	V	G	L	Y	L	A
60



V	C	V	G	G	L	L	G	N	C
70



L	V	M	Y	V	I	L	R	H	T
80


ICL1				TM2
K	M	K	T	A	T	N	I	Y	I
90



F	N	L	A	L	A	D	T	L	V
100



L	L	T	L	P	F	Q	G	T	D
110


				ECL1
I	L	L	G	F	W	P	F	G	N
120


TM3
A	L	C	K	T	V	I	A	I	D
130



Y	Y	N	M	F	T	S	T	F	T
140



L	T	A	M	S	V	D	R	Y	V
150


				ICL2
A	I	C	H	P	I	R	A	L	D
160


		TM4
V	R	T	S	S	K	A	Q	A	V
170



N	V	A	I	W	A	L	A	S	V
180



V	G	V	P	V	A	I	M	G	S
190


ECL2
A	Q	V	E	D	E	E	I	E	C
200


						TM5
L	V	E	I	P	T	P	Q	D	Y
210



W	G	P	V	F	A	I	C	I	F
220



L	F	S	F	I	V	P	V	L	V
230



I	S	V	C	Y	S	L	M	I	R
240


					ICL3
R	L	R	G	V	R	L	L	S	G
250


TM6
S	R	E	K	D	R	N	L	R	R
260



I	T	R	L	V	L	V	V	V	A
270



V	F	V	G	C	W	T	P	V	Q
280



V	F	V	L	A	Q	G	L	G	V
290


ECL3				TM7
Q	P	S	S	E	T	A	V	A	I
300



L	R	F	C	T	A	L	G	Y	V
310



N	S	C	L	N	P	I	L	Y	A
320


		H8
F	L	D	E	N	F	K	A	C	F
330



R	K	F	C	C	A	S	A	L	R
340


C-term
R	D	V	Q	V	S	D	R	V	R
350



S	I	A	K	D	V	A	L	A	C
360



K	T	S	E	T	V	P	R	P	A
370


hemagglutin


FLAG


BRIL (b562R


	N-term						TM1
	G	A	F	L	P	L	G	L	K
51



V	T	I	V	G	L	Y	L	A	V
61



C	V	G	G	L	L	G	N	C	L
71



V	M	Y	V	I	L	R	H	T	K
81


ICL1				TM2
M	K	T	A	T	N	I	Y	I	F
91



N	L	A	L	A	D	T	L	V	L
101



L	T	L	P	F	Q	G	T	D	I
111


			ECL1				TM3
L	L	G	F	W	P	F	G	N	A
121



L	C	K	T	V	I	A	I	D	Y
131



Y	N	M	F	T	S	T	F	T	L
141



T	A	M	S	V	D	R	Y	V	A
151


			ICL2
I	C	H	P	I	R	A	L	D	V
161


	TM4
R	T	S	S	K	A	Q	A	V	N
171



V	A	I	W	A	L	A	S	V	V
181



G	V	P	V	A	I	M	G	S	A
191


ECL2
Q	V	E	D	E	E	I	E	C	L
201


					TM5
V	E	I	P	T	P	Q	D	Y	W
211



G	P	V	F	A	I	C	I	F	L
221



F	S	F	I	V	P	V	L	V	I
231



S	V	C	Y	S	L	M	I	R	R
241


				ICL3
L	R	G	V	R	L	L	S	G	S
251


TM6
R	E	K	D	R	N	L	R	R	I
261



T	R	L	V	L	V	V	V	A	V
271



F	V	G	C	W	T	P	V	Q	V
281



F	V	L	A	Q	G	L	G	V	Q
291


ECL3				TM7
P	S	S	E	T	A	V	A	I	L
301



R	F	C	T	A	L	G	Y	V	N
311



S	C	L	N	P	I	L	Y	A	F
321


	H8
L	D	E	N	F	K	A	C	F	R
331


				C-term
K	F	C	C	A	S	A	L


PreScission


His(10)

SCHEMATIC

Perspective: Wild-type Construct

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

ICL3

TM6

ECL3

TM7

H8

C-term

hemagg

FLAG

BRIL (

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

ICL3

TM6

ECL3

TM7

H8

C-term

PreSci

His(10

DIAGRAMS

MUTATIONS

Position	Wild-type	Mutated

DELETIONS

from 1 to 42

from 340 to 370

MODIFICATIONS

AUXILIARY PROTEINS

Protein_type: signal

Name: hemagglutinin

Sequence: None

Deletions:

Positions: N-term_0 None:None

Presence in Crystal:NO

Remarks: None

Protein_type: tag

Name: FLAG

Sequence: None

Deletions:

Positions: N-term_1 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: fusion

Name: BRIL (b562RIL)

Sequence: None

Deletions:

Positions: N-term_2 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: prot_cleavage

Name: PreScission site

Sequence: None

Deletions:

Positions: C-term_3 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: tag

Name: His(10)

Sequence: None

Deletions:

Positions: C-term_4 None:None

Presence in Crystal:YES

Remarks: None

EXPRESSION

Expression method	Host cell type	Host cell	Remarks
Baculovirus	Sf9	Insect	pFastBac1 and Bac-to-Bac

SOLUBILIZATION

Solvent type	Item	Concentration
additive	CHS	0.1 %w/v
detergent	DDM	0.5 %w/v

Remarks:

PURIFICATION

Purification type	Description
Alkylation by Iodoacetamide	None

Remarks:None

CRYSTALLIZATION

METHOD: in meso/LCP

TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))

PROTEIN CONCENTRATION: 40 mg/ml

Temperature: 20°C

ph : 6.4-6.4

Remarks:

Chemical lists

Type	Item	Concentration
Additional
unknown	PEG 400	25-30 % v/v
unknown	potassium sodium tartrate tetrahydrate	100-200 mM
unknown	Bis-tris propane pH 6.4	100 mM
Detergent
unknown	DDM	0.025 %w/v
Lipid
unknown	CHS	0.005 %w/v